Mouse CD45 ELISA Kit KE10140 proteintech
$299.00
In stock
SKU
KE10140
| Product name | Mouse CD45 ELISA Kit |
| Synonyms | Cd45,Ptprc,B220,LCA,Leukocyte common antigen |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 19264 |
| Sample Type | Average Range |
| Cell culture supernatant | 97% 96%-99% |
| Cell lysate | 100% 98%-104% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 235.6 11.9 5.1 |
| 2 | 24 57.2 2.5 4.4 |
| 3 | 24 15.3 1.0 6.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets. CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Cd45,Ptprc,B220,LCA,Leukocyte common antigen FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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