Mouse CD47 ELISA Kit KE10226 proteintech
$299.00
In stock
SKU
KE10226
| Product name | Mouse CD47 ELISA Kit |
| Synonyms | IAP, Integrin-associated protein, Leukocyte surface antigen CD47 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16423 |
| Sample Type | Average Range |
| Mouse serum | 110% 106%-115% |
| Lysate | 102% 84%-115% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.60 0.32 5.69 |
| 2 | 16 1.34 0.06 4.53 |
| 3 | 16 0.40 0.02 5.24 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD47, also known as MER6, is a cell surface glycoprotein molecule belonging to the immunoglobulin superfamily. It is a key tumor antigen involved in the development and progression of various cancers. CD47 is a ligand for SIRPα, a protein expressed on macrophages and dendritic cells. The interaction of CD47 with SIRPα triggers a "don't eat me" signal to the macrophages, inhibiting phagocytosis. Additionally, the CD47-SIRPα axis is a key immune checkpoint in different cancers including hematological malignancies. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms IAP, Integrin-associated protein, Leukocyte surface antigen CD47 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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