Mouse CD73 ELISA Kit KE10174 proteintech
$299.00
In stock
SKU
KE10174
| Product name | Mouse CD73 ELISA Kit |
| Synonyms | 5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 78.1-5000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 23959 |
| Sample Type | Average Range |
| Lysate | 100% 97%-101% |
| Tissue homogenates | 100% 97%-101% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,269.5 128.0 5.6 |
| 2 | 16 561.0 20.1 3.6 |
| 3 | 16 170.9 5.7 3.3 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD73 is a membrane-bound enzyme that catalyzes the extracellular hydrolysis of adenosine monophosphate (AMP) to produce adenosine. It is expressed on various immune cells, including T cells, B cells, dendritic cells, and regulatory T cells (Tregs). CD73 works in conjunction with CD39 to break down extracellular ATP into adenosine, creating an immunosuppressive environment that can inhibit the function of cytotoxic T cells and natural killer (NK) cells. This process is essential for immune tolerance and the resolution of inflammation. CD73 is also an important player in controlling tumor progression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms 5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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