| Product name | Mouse DPP4/CD26 ELISA Kit |
| Synonyms | CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.39-25 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13482 |
| Sample Type | Average Range |
| Mouse serum | 77% 74%-80% |
| Mouse urine | 88% 76%-101% |
| Cell culture supernatant | 78% 76%-80% |
| Tissue lysate | 90% 85%-97% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 13.43 0.60 4.49 |
| 2 | 16 3.21 0.16 5.04 |
| 3 | 16 1.00 0.06 5.80 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Dipeptidyl peptidase 4 (DPP4, also known as CD26) is a type II transmembrane glycoprotein with enzymatic activity. DPP4 plays a crucial role in immune regulation, T-cell activation, and the regulation of chemokine function. DPP4 also interacts with adenosine deaminase (ADA) and the chemokine receptor CXCR4, influencing immune responses and cell migration. Additionally, circulating soluble DPP4 (sDPP4) is released from adipose tissue and acts as a pro-inflammatory adipokine, linking the expansion of adipose tissue with increased inflammation, insulin resistance, and metabolic syndrome. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 membrane form, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.