Mouse HER2/ErbB2 ELISA Kit KE10168 proteintech
$299.00
In stock
SKU
KE10168
| Product name | Mouse HER2/ErbB2 ELISA Kit |
| Synonyms | CD340, EC:2.7.10.1, Erbb2, Her2, Her2 / ErbB2 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13866 |
| Sample Type | Average Range |
| Cell lysate | 105% 96%-125% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.59 0.22 3.88 |
| 2 | 16 1.33 0.10 7.17 |
| 3 | 16 0.73 0.03 3.88 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
HER2/ErbB2 is a critical receptor tyrosine kinase protein belonging to the epidermal growth factor receptor (EGFR) family, which also includes HER1 (EGFR), HER3, and HER4. Unlike other members of the EGFR family, HER2/ErbB2 does not have a known ligand and is often referred to as an "orphan receptor." However, it can form homodimers or heterodimers with other EGFR family members, such as HER1, HER3, and HER4, significantly enhancing its signaling potential. This receptor is involved in the regulation of multiple downstream signaling pathways, including the PI3K-Akt and MAPK pathways, which promote cell survival, proliferation, and migration. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD340, EC:2.7.10.1, Erbb2, Her2, Her2 / ErbB2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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