Mouse IL-12RB1 ELISA Kit KE10217 proteintech
$299.00
In stock
SKU
KE10217
| Product name | Mouse IL-12RB1 ELISA Kit |
| Synonyms | CD212, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, Il12rb |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.78-50 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16161 |
| Sample Type | Average Range |
| Tissue lysate | 115% 107%-126% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 25.95 1.77 6.82 |
| 2 | 16 6.19 0.46 7.48 |
| 3 | 16 3.12 0.15 4.88 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD212, IL-12 receptor beta component, IL-12 receptor subunit beta-1, IL-12R subunit beta-1, Il12rb FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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