Mouse LAG-3/CD223 ELISA Kit KE10189 proteintech
$299.00
In stock
SKU
KE10189
| Product name | Mouse LAG-3/CD223 ELISA Kit |
| Synonyms | CD223, LAG-3, LAG 3, Lag3, Ly66 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.10 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16768 |
| Sample Type | Average Range |
| Cell culture supernatant | 95% 80%-119% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 12.29 0.90 7.31 |
| 2 | 16 2.42 0.07 2.92 |
| 3 | 16 1.31 0.03 2.63 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. Its soluble form, sLAG-3, produced via alternative splicing and proteolytic cleavage of the extracellular domain, is a natural high-affinity ligand for MHC class II. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD223, LAG-3, LAG 3, Lag3, Ly66 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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