| Product name | Mouse LIF ELISA Kit |
| Synonyms | leukemia inhibitory factor, Lif |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.9 pg/mL |
| Range | 3.9-250 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16878 |
| Sample Type | Average Range |
| Cell culture supernatants | 105% 81%-124% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 117.0 5.3 4.5 |
| 2 | 24 28.1 1.1 4.0 |
| 3 | 24 6.9 0.4 6.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms leukemia inhibitory factor, Lif FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.