Mouse Mesothelin ELISA Kit KE10222 proteintech
$299.00
In stock
SKU
KE10222
| Product name | Mouse Mesothelin ELISA Kit |
| Synonyms | Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 56047 |
| Sample Type | Average Range |
| Mouse serum | 97% 90%-106% |
| Cell lysate | 91% 81%-100% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 10.50 1.08 10.33 |
| 2 | 16 2.58 0.26 10.19 |
| 3 | 16 0.68 0.08 12.54 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Mesothelin is a 40-kDa cell surface glycoprotein that is present on normal mesothelial cells lining the pleura, peritoneum and pericardium. Mesothelin expression in normal human tissues is observed only in a single layer of mesothelial cells lining the pleura, peritoneum and pericardium, surface epithelial cells of the ovary, tunica vaginalis, rete testis and the tonsilar and fallopian tube epithelial cells. However, mesothelin is highly expressed in several cancers, including epitheloid mesotheliomas, pancreatic, biliary adenocarcinomas, gastric and ovarian cancers. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Megakaryocyte-potentiating factor, Mes, Mesothelin, cleaved form, Mpf, Msln FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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