Mouse P62/SQSTM1 ELISA Kit KE10163 proteintech
$299.00
In stock
SKU
KE10163
| Product name | Mouse P62/SQSTM1 ELISA Kit |
| Synonyms | p62, Sqstm1, OSF 6, Osi, sequestosome 1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 0.39-25 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18412 |
| Sample Type | Average Range |
| Cell lysate | 114% 103%-119% |
| Tissue lysate | 88% 85%-93% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 22.31 1.82 8.15 |
| 2 | 16 5.49 0.47 8.56 |
| 3 | 16 2.77 0.23 8.39 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Sequestosome 1 (P62/SQSTM1) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. P62 has been implicated in shuttling ubiquitinated and aggregated proteins for autophagic degradation. P62 is degraded during the autophagic process, which makes its intracellular level a marker for autophagy progression. P62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. P62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease, and nephropathic cystinosis. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms p62, Sqstm1, OSF 6, Osi, sequestosome 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
