Mouse Serpin E1/PAI-1 ELISA Kit KE10137 proteintech
$299.00
In stock
SKU
KE10137
| Product name | Mouse Serpin E1/PAI-1 ELISA Kit |
| Synonyms | PAI-1, Serpine1, PAI 1, PAI1, Plasminogen activator inhibitor 1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 9.8-625 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 18787 |
| Sample Type | Average Range |
| Cell culture supernatant | 109% 107%-113% |
| Tissue lysate | 114% 109%-120% |
| Mouse serum | 98% 84%-105% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 320.7 11.4 3.6 |
| 2 | 24 78.5 3.5 4.4 |
| 3 | 24 18.3 1.5 8.3 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Serpin E1, also known as plasminogen activator inhibitor 1 (PAI-1), is a protein that belongs to the serpin family of serine protease inhibitors. It is primarily produced by endothelial cells and plays a crucial role in the regulation of fibrinolysis by inhibiting the activity of plasminogen activators, such as tissue plasminogen activator (tPA) and urokinase (uPA). Serpin E1 is involved in a variety of physiological and pathological processes, including fibrinolysis, fibrosis, angiogenesis, wound healing, and the invasion and metastasis of tumor cells. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms PAI-1, Serpine1, PAI 1, PAI1, Plasminogen activator inhibitor 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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