Mouse MIF ELISA Kit KE10027 proteintech
$299.00
In stock
SKU
KE10027
| Product name | Mouse MIF ELISA Kit |
| Synonyms | GIF, Glif, Mif |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 17319 |
| Sample Type | Average Range |
| Cell culture supernatants | 109% 86%-119% |
| Mouse serum | 104% 87%-117% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 40.6 2.7 6.5 |
| 2 | 24 391.2 25.0 6.3 |
| 3 | 24 1,645.0 107.9 6.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| mouse | Serum, Plasma, Cell culture supernatants Exp Eye Res The CD74-MIF axis contributes to acute uveitic injury by upregulating proinflammatory cytokine secretion in murine cytomegalovirus intracameral infection mouse models. Authors - Qilian Sheng View Article |
| FH Laura (Verified Customer) (11-12-2025) | Mouse MIF ELISA kit was used to dose MIF in supernatant from melanoma cells in vitro, from tumor secretome and from mice plasma. For our study, the samples needed to be diluted 1:25 (culture cells and plasma) to 1:300 (tumor secretome) Applications: Other |
Macrophage migration inhibitory factor (MIF) is an upstream regulator of the innate and adaptive immune response that is widely expressed and that exhibits chemokine-like activities. MIF play a role in the pathogenesis of inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, sepsis, asthma, and acute respiratory distress syndrome. MIF is expressed by the ischemic myocardium and that it may exhibit an overall cardioprotective role during I/R injury. MIF regulates inflammatory processes by recruiting immune cells, amplifying cytokine production, inhibiting apoptosis and counter-regulating the immunosuppressive action of glucocorticoids. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms GIF, Glif, Mif FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Myeloid cell-derived apCAFs promote HNSCC progression by regulating proportion of CD4+ and CD8+ T cells
Journal: J Exp Clin Cancer Res | Authors: Feilong Ren | Species: mouse | Application: serum - Obacunone targets macrophage migration inhibitory factor (MIF) to impede osteoclastogenesis and alleviate ovariectomy-induced bone loss
Journal: J Adv Res | Authors: Jianbo He | Species: mouse | Application: serum - VE-cadherin regulates migration inhibitory factor synthesis and release.
Journal: Inflamm Res | Authors: Ranran Li | Species: mouse | Application: plasma - Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI.
Journal: Cell Death Discov | Authors: Tianlong Li | Species: mouse | Application: serum - Bacillus Subtilis-Derived Exopolysaccharide Halts Depigmentation and Autoimmunity in Vitiligo
Journal: J Invest Dermatol | Authors: Ahmed A Touni | Species: mouse | Application: serum - The CD74-MIF axis contributes to acute uveitic injury by upregulating proinflammatory cytokine secretion in murine cytomegalovirus intracameral infection mouse models.
Journal: Exp Eye Res | Authors: Qilian Sheng | Species: mouse | Application: Serum, Plasma, Cell culture supernatants - Bacillus Subtilis-Derived Exopolysaccharide Halts Depigmentation and Autoimmunity in Vitiligo
Journal: J Invest Dermatol | Authors: Ahmed A Touni | Species: mouse - VE-cadherin regulates migration inhibitory factor synthesis and release.
Journal: Inflamm Res | Authors: Ranran Li | Species: mouse - S-adenosylhomocysteine hydrolase-like protein 1 deficiency resulted in stronger inflammation reaction in acute pancreatitis.
Journal: J Gastroenterol | Authors: Juan Xiao | Species: mouse - Macrophage migration inhibitory factor (MIF) inhibitor 4-IPP suppresses osteoclast formation and promotes osteoblast differentiation through the inhibition of the NF-κB signaling pathway.
Journal: FASEB J | Authors: Lin Zheng | Species: mouse - Macrophage migration inhibitory factor: A noval biomarker upregulates in myasthenia gravis and correlates with disease severity and relapse
Journal: Cytokine | Authors: Xiaoyu Huang | Species: mouse - The CD74-MIF axis contributes to acute uveitic injury by upregulating proinflammatory cytokine secretion in murine cytomegalovirus intracameral infection mouse models.
Journal: Exp Eye Res | Authors: Qilian Sheng | Species: mouse
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
