Mouse TNFR1 ELISA Kit KE10065 proteintech
$299.00
In stock
SKU
KE10065
| Product name | Mouse TNFR1 ELISA Kit |
| Synonyms | CD120a, FPF, p55, p55 R, TNF alphaR1, TNF R, TNF R I, TNF R1, TNF R55, TNFalpha R1, TNFAR, Tnfr 2, Tnfr1, TNFR60, TNFRI, TNFRp55, Tnfrsf1a |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 21937 |
| Sample Type | Average Range |
| Mouse serum | 98% 80%-123% |
| Cell culture supernates | 96% 71%-124% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 205.8 7.4 3.6 |
| 2 | 24 51.9 2.1 4.0 |
| 3 | 24 12.5 0.6 4.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55-kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD120a, FPF, p55, p55 R, TNF alphaR1, TNF R, TNF R I, TNF R1, TNF R55, TNFalpha R1, TNFAR, Tnfr 2, Tnfr1, TNFR60, TNFRI, TNFRp55, Tnfrsf1a FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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