Mouse/Rat TREM-1 ELISA Kit KE10080 proteintech
$299.00
In stock
SKU
KE10080
| Product name | Mouse/Rat TREM-1 ELISA Kit |
| Synonyms | Trem1 |
| Reactivity | Mouse/Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 0.4 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 58217 |
| Sample Type | Average Range |
| Cell culture supernatants | 92% 79%-108% |
| Mouse serum | 88% 70%-130% |
| Rat serum | 86% 70%-118% |
| Tissue lysates | 115% 99%-125% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 428.5 13.0 3.0 |
| 2 | 24 102.8 2.0 1.9 |
| 3 | 24 26.5 1.0 3.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Triggering receptor expressed on myeloid cells 1 (TREM-1, also known as CD354) is a member of the immunoglobulin superfamily, which is expressed on neutrophils and monocytes. TREM1 plays a role in the innate immune system. TREM1 amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. TREM1 can also exist as a soluble form (sTREM1). Increased plasma level of sTREM1 have been reported in specific diseases including Alzheimer's disease, systemic sepsis, acute myocardial infarction and subarachnoid hemorrhage. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Trem1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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