Rat CD4 ELISA Kit KE20041 proteintech
$299.00
In stock
SKU
KE20041
| Product name | Rat CD4 ELISA Kit |
| Synonyms | CD4 molecule, p55 |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 10.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24932 |
| Sample Type | Average Range |
| Tissue lysate | 95% 85%-114% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,975.1 162.2 8.2 |
| 2 | 16 530.8 29.4 5.5 |
| 3 | 16 162.8 11.1 6.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD4 is a transmembrane glycoprotein of the immunoglobulin superfamily (IgSF), containing four extracellular Ig-like domains, a hydrophobic transmembrane region, and a 40-residue cytoplasmic tail. CD4 is expressed in T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It is involved in T cell differentiation, development, and activation by interacting with antigen-presenting cells (APCs). The cytoplasmic tail of CD4 is associated with the Lck kinase, which in turn activates the signaling components of the T cell receptor (TCR)-CD3 complexes. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD4 molecule, p55 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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