Rat Lipocalin-2/NGAL ELISA Kit KE20034 proteintech
$299.00
In stock
SKU
KE20034
| Product name | Rat Lipocalin-2/NGAL ELISA Kit |
| Synonyms | Lcn2,Alpha-2-microglobulin-related protein,Alpha-2 |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.8 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 170496 |
| Sample Type | Average Range |
| Rat serum | 103% 98%-109% |
| Tissue lysate | 105% 99%-111% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 136.3 3.4 2.5 |
| 2 | 24 49.0 0.7 1.5 |
| 3 | 24 21.7 0.6 2.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Lcn2,Alpha-2-microglobulin-related protein,Alpha-2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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