Speedy™ Human CD69 One-Step ELISA Kit SE50109 proteintech
$449.00
In stock
SKU
SE50109
| Product name | Speedy™ Human CD69 One-Step ELISA Kit |
| Synonyms | Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.9 pg/mL |
| Range | 23.4-1500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 969 |
| Sample Type | Average Range |
| Human plasma | 104% 97%-114% |
| Cell lysate | 110% 102%-118% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 546.9 17.2 3.1 |
| 2 | 16 138.0 7.1 5.1 |
| 3 | 16 64.9 2.5 3.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD69 is a type II C-type lectin receptor that serves as an early activation marker for lymphocytes. It is rapidly expressed on the surface of T cells, B cells, and natural killer (NK) cells following activation. CD69 plays a crucial role in lymphocyte proliferation and functions as a signal-transmitting receptor, influencing cell cycle progression and cytokine production. It also regulates the differentiation of T helper (Th) subsets, including Th1, Th2, Th17, and regulatory T cells (Tregs), by modulating intracellular signaling pathways and cytokine secretion. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Activation inducer molecule, AIM, BL AC/P26, BL-AC/P26, CD69 molecule FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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