Speedy™ Human EPCAM/CD326 One-Step ELISA Kit SE50113 proteintech
$449.00
In stock
SKU
SE50113
| Product name | Speedy™ Human EPCAM/CD326 One-Step ELISA Kit |
| Synonyms | EPCAM, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, CO 17A |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 5.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4072 |
| Sample Type | Average Range |
| Human serum | 103% 93%-108% |
| Urine | 79% 77%-81% |
| Cell culture supernatant | 106% 93%-122% |
| Cell lysate | 110% 99%-123% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 521.2 13.2 2.5 |
| 2 | 16 130.8 3.6 2.8 |
| 3 | 16 36.6 0.9 2.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Epithelial cell adhesion molecule (EPCAM), also known as CD326, is a well-characterized type I transmembrane glycoprotein that plays a pivotal role in cell adhesion, proliferation, differentiation, and signaling. It mediates Ca2+-independent homotypic cell-cell adhesion through its extracellular domain, which is crucial for the formation and maintenance of epithelial tissues. The membrane localization of EPCAM is essential for its function in cell adhesion and signaling. Additionally, elevated levels of soluble EPCAM have been associated with tumor burden and poor prognosis in cancer patients, making it a potential biomarker for cancer diagnosis and monitoring. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms EPCAM, Adenocarcinoma-associated antigen, CD326, Cell surface glycoprotein Trop-1, CO 17A FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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