| Product name | Mouse CCL5 ELISA Kit |
| Synonyms | C-C motif chemokine 5, MuRantes, RANTES, Scya5, SIS-delta |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma,Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 5.7 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20304 |
| Sample Type | Average Range |
| Mouse serum | 87% 74%-124% |
| Cell culture supernatants | 106% 83%-121% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 55.8 5.3 9.5 |
| 2 | 24 259.1 23.8 9.2 |
| 3 | 24 905.6 58.1 6.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| mouse | cell culture supernatants Clin Cancer Res Targeting Dendritic Cell Dysfunction to Circumvent Anti-PD1 Resistance in Head and Neck Cancer Authors - Shin Saito View Article |
CCL5, also known as RANTES, belongs to the CC chemokine family. It is strongly expressed in the human and murine liver upon injury. CCL5 has been found to be produced by numerous cell types, including platelets, macrophages, endothelial, and stellate cells. CCL5 recruits T cells, dendritic cells, eosinophils, NK cells, mast cells, and basophils to sites of inflammation by interacting with 3 specific G protein-coupled receptors: CCR1, CCR3, and CCR5. While CCL5 may promote efficient anti-tumour immune responses, it has also been associated with cancer progression and metastasis. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms C-C motif chemokine 5, MuRantes, RANTES, Scya5, SIS-delta FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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