Anti-SARS-CoV-2 S-RBD protein Human IgM ELISA Kit KE30004 proteintech
$399.00
In stock
SKU
KE30004
| Product name | Anti-SARS-CoV-2 S-RBD protein Human IgM ELISA Kit |
| Synonyms | COVID-19 RBD, RBD, Spike, spike protein, S protein |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Indirect |
| Sensitivity | N/A |
| Range | 1.56-100 ng/mL |
| Tested applications | Indirect ELISA |
| Gene ID (NCBI) | 43740568 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | serum J Med Virol Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines Authors - Xuan Yi View Article |
A promising target for both diagnosis and therapeutics treatments of the new disease named COVID-19 is the coronavirus (CoV) spike (S) glycoprotein. The spike protein, which is responsible for the "corona" (Latin word for crown) appearance in all coronaviruses, is a type I glycoprotein that has an especial role in the interaction between the virus and the host cell. This protein attaches itself to specific cellular receptors and suffers a conformational change that enables the fusion of the virus and the cell. Studies have shown that the SARS-CoV-2's S RBD protein interacts strongly with the Angiotensin-converting enzyme 2 (ACE2). S-RBD protein in order to enlighten the binding epitopes of these Abs. Because of the conservation of S-RBD protein sequence and its strong immunogenicity, the S-RBD protein of coronavirus is chosen as a diagnostic tool. COVID-19 antibodies can be produced by a host immune system following exposure to SARS-CoV-2. IgM and IgG antibodies are also known as immunoglobulins M and G, respectively, and are among the antibody isotypes produced by vertebrate immune systems. The ELISA microplate is coated with the SARS-CoV-2 S-RBD protein. The coated S-RBD protein binds with COVID-19 IgM S-RBD antibodies in the serum sample. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms COVID-19 RBD, RBD, Spike, spike protein, S protein FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- SARS-CoV-2 nucleocapsid protein, rather than spike protein, triggers a cytokine storm originating from lung epithelial cells in patients with COVID-19
Journal: Infection | Authors: Ying-Chuan Wang | Species: human | Application: serum - RBD-specific antibody response after two doses of different SARS-CoV-2 vaccines during the mass vaccination campaign in Mongolia
Journal: PLoS One | Authors: Burenjargal Batmunkh | Species: human | Application: serum - Longitudinal Analyses after COVID-19 Recovery or Prolonged Infection Reveal Unique Immunological Signatures after Repeated Vaccinations
Journal: Vaccines (Basel) | Authors: Daisuke Hisamatsu | Application: serum - Dynamic Changes in Antibodies and Proteome in Breast Milk of Mothers Infected with Wild-Type SARS-CoV-2 and Omicron: A Longitudinal Study
Journal: Nutrients | Authors: Yaqiong Guo | Species: human | Application: milk - PD-1(high)CXCR5(-)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
Journal: Cell Rep | Authors: Hiromitsu Asashima | Species: human | Application: serum - Pre-existing immunity to SARS-CoV-2 associates with strong T cell responses induced by inactivated COVID-19 vaccines
Journal: J Med Virol | Authors: Xuan Yi | Species: human | Application: serum
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