Speedy™ Human IL7Ra/CD127 One-Step ELISA Kit SE50131 proteintech
$449.00
In stock
SKU
SE50131
| Product name | Speedy™ Human IL7Ra/CD127 One-Step ELISA Kit |
| Synonyms | CD127, IL-7Ra/CD127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 6.1 pg/mL |
| Range | 19.5-1250 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3575 |
| Sample Type | Average Range |
| Human plasma | 108% 99%-127% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 615.7 19.3 3.1 |
| 2 | 16 167.9 3.7 2.2 |
| 3 | 16 91.0 1.6 1.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed in thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD127, IL-7Ra/CD127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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