Speedy™ Human PAK4 One-Step ELISA Kit SE50150 proteintech
$449.00
In stock
SKU
SE50150
| Product name | Speedy™ Human PAK4 One-Step ELISA Kit |
| Synonyms | EC:2.7.11.1, KIAA1142, p21-activated kinase 4, PAK 4, PAK-4 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.26 ng/mL |
| Range | 0.625-40 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10298 |
| Sample Type | Average Range |
| Cell lysate | 113% 103%-120% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 21.87 0.65 2.97 |
| 2 | 16 5.68 0.11 1.94 |
| 3 | 16 3.28 0.10 3.05 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
PAK4 (p21-activated kinase 4) is a serine/threonine kinase that plays a crucial role in regulating various cellular processes, including cell proliferation, survival, migration, and cytoskeletal dynamics. PAK4 is involved in several signaling pathways that are important for cellular homeostasis and development. Dysregulation of PAK4 activity has been implicated in various diseases, particularly cancer, where it contributes to tumorigenesis by promoting cell survival and migration. Additionally, PAK4 is thought to participate in the regulation of neurogenesis and synaptic plasticity. Given its pivotal role in cell signaling and disease, PAK4 has become a potential target for therapeutic interventions in cancer and other disorders. Inhibition of PAK4 has been shown to impair tumor growth in preclinical models, making it a promising candidate for drug development. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms EC:2.7.11.1, KIAA1142, p21-activated kinase 4, PAK 4, PAK-4 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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