Speedy™ Human PTPRS One-Step ELISA Kit SE50183 proteintech
$449.00
In stock
SKU
SE50183
| Product name | Speedy™ Human PTPRS One-Step ELISA Kit |
| Synonyms | EC:3.1.3.48, PTPSIGMA, R PTP S, R PTP sigma, Receptor-type tyrosine-protein phosphatase S |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Human cerebrospinal fluid(CSF) |
| Assay type | Sandwich |
| Sensitivity | 3.9 pg/mL |
| Range | 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5802 |
| Sample Type | Average Range |
| Human plasma | 108% 104%-114% |
| Human cerebrospinal fluid (CSF) | 101% 99%-104% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,142.7 61.6 2.9 |
| 2 | 16 469.8 9.7 2.1 |
| 3 | 16 228.4 4.1 1.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair. The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants. Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ, and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa. rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80 kDa immunoreactive band. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms EC:3.1.3.48, PTPSIGMA, R PTP S, R PTP sigma, Receptor-type tyrosine-protein phosphatase S FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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