Speedy™ Human RNASET2 One-Step ELISA Kit SE50081 proteintech
$449.00
In stock
SKU
SE50081
| Product name | Speedy™ Human RNASET2 One-Step ELISA Kit |
| Synonyms | RNase T2, bA514O12.3, EC:4.6.1.19, Ribonuclease 6, Ribonuclease T2 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 8635 |
| Sample Type | Average Range |
| Human plasma | 99% 85%-108% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 4.82 0.10 2.07 |
| 2 | 16 1.31 0.04 3.05 |
| 3 | 16 0.42 0.02 4.76 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
RNASET2 (Ribonuclease T2) is a highly conserved enzyme in a wide range of organisms and belongs to the RNase T2 family. The main biological role of intracellular RNASET2 is to cleave or degrade exogenous or endogenous RNA substrates as well as contributing to mitochondrial RNA metabolism, and also activating immune responses. In addition, it is involved in the regulation of angiogenesis, apoptosis, cell proliferation/differentiation, and immune regulation. In cancer, dysregulated expression of RNASET2 is associated with a variety of pathological conditions, including autoimmune diseases and cancer. The calculated molecular weight of human RNASET2 is approximately 35 kDa, but due to post-translational modifications such as glycosylation, its actual molecular weight may be observed as a mildly glycosylated form with a molecular weight of 38-45 kDa and a highly glycosylated form with a molecular weight of 50-80 kDa. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms RNase T2, bA514O12.3, EC:4.6.1.19, Ribonuclease 6, Ribonuclease T2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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