| Product name | Human EGF ELISA Kit |
| Synonyms | EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 26.9 pg/mL |
| Range | 125-8000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1950 |
| Sample Type | Average Range |
| Human plasma | 88% 71%-110% |
| Human serum | 92% 71%-112% |
| Cell culture supernatants | 101% 80%-123% |
| Saliva | 102% 79%-127% |
| Urine | 108% 90%-125% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 583.4 37.5 6.4 |
| 2 | 24 2,306.4 182.9 7.9 |
| 3 | 24 7,538.8 608.2 8.1 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | cell culture supernatants Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma Authors - Minghao Xu View Article |
Epidermal growth factor (EGF) is a member of the epidermal growth factor superfamily. EGF preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. EGF binds to the EGF receptor on the surface of cells and mediates intrinsic phosphorylation of the receptor on tyrosine residues. It has been detected in nearly all body fluids, such as urine (urogastrone), saliva, milk and platelet-rich plasma. EGF plays important roles in multiple biological processes, such as the regulation of cell growth, proliferation, and differentiation. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms EGF, HOMG4, Pro epidermal growth factor, URG, Urogastrone FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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