Speedy™ Mouse Endoglin/CD105 One-Step ELISA Kit SE60024 proteintech
$449.00
In stock
SKU
SE60024
| Product name | Speedy™ Mouse Endoglin/CD105 One-Step ELISA Kit |
| Synonyms | CD105, Edg, endoglin, Eng, S endoglin |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 9.4 pg/mL |
| Range | 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13805 |
| Sample Type | Average Range |
| Mouse serum | 116% 87%-125% |
| Cell culture supernatant | 93% 88%-100% |
| Tissue lysate | 92% 89%-95% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,032.2 54.1 2.7 |
| 2 | 16 511.8 13.6 2.7 |
| 3 | 16 148.5 5.2 3.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Endoglin (CD105) is a homodimeric cell membrane glycoprotein that acts as an accessory receptor for transforming growth factor-beta (TGF-β) and is involved in vascular development and remodeling. It is highly expressed on vascular endothelial cells and plays a crucial role in angiogenesis, making it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. Endoglin is also involved in the regulation of vascular endothelial cell migration and has important implications for embryonic heart development. Additionally, it can regulate the response of endothelial cells to blood flow, influencing vascular remodeling and the establishment of normal vascular morphology during angiogenesis. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD105, Edg, endoglin, Eng, S endoglin FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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