Speedy™ Mouse E-selectin/CD62E One-Step ELISA Kit SE60021 proteintech
$449.00
In stock
SKU
SE60021
| Product name | Speedy™ Mouse E-selectin/CD62E One-Step ELISA Kit |
| Synonyms | CD62E, E-selectin, E selectin, Elam, Sele |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 41.2 pg/mL |
| Range | 125-8000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20339 |
| Sample Type | Average Range |
| Mouse serum | 102% 80%-116% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 4,569.9 243.5 5.3 |
| 2 | 16 1,179.9 50.8 4.3 |
| 3 | 16 683.2 19.3 2.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also include L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD62E, E-selectin, E selectin, Elam, Sele FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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