Speedy™ Mouse IL-13RA2 One-Step ELISA Kit SE60011 proteintech
$449.00
In stock
SKU
SE60011
| Product name | Speedy™ Mouse IL-13RA2 One-Step ELISA Kit |
| Synonyms | CD213a2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, Il13ra2, IL-13R-alpha-2 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16165 |
| Sample Type | Average Range |
| Mouse serum | 96% 94%-98% |
| Tissue homogenates | 92% 88%-97% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.13 0.17 3.31 |
| 2 | 16 1.26 0.03 2.38 |
| 3 | 16 0.64 0.01 1.56 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
IL-13RA2, also known as IL-13R and CD213a2, belongs to the type I cytokine receptor family. It binds as a monomer with high affinity to interleukin-13 (IL-13), but not to interleukin-4 (IL-4). IL-13RA2 lacks the cytoplasmic domain for signaling, indicating that it acts as a decoy receptor. IL13RA2 polymorphisms have been associated with systemic sclerosis. Overexpression of IL-13RA2 has been reported in several types of cancer, including melanoma, lung cancer, and brain tumor, which makes it a potential immunotherapeutic target. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD213a2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, Il13ra2, IL-13R-alpha-2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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