Speedy™ Mouse IL-4 R alpha/CD124 One-Step ELISA Kit SE60045 proteintech
$449.00
In stock
SKU
SE60045
| Product name | Speedy™ Mouse IL-4 R alpha/CD124 One-Step ELISA Kit |
| Synonyms | CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha, Il4ra |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 5.4 pg/mL |
| Range | 23.4-1500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16190 |
| Sample Type | Average Range |
| Mouse serum | 96% 81%-112% |
| Tissue lysate | 98% 88%-113% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 805.9 20.6 2.6 |
| 2 | 16 212.9 3.3 1.6 |
| 3 | 16 114.9 2.3 2.0 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
IL-4Rα (IL-4 receptor alpha chain, also known as CD124) is an important member of the cytokine receptor family, primarily involved in immune responses and cellular signal transduction. It is a single-pass transmembrane protein that binds to IL-4 and IL-13 through its extracellular domain, activating the JAK/STAT signaling pathway to regulate cell proliferation, differentiation, and function. IL-4Rα is expressed in various immune cells (such as T cells, B cells, and macrophages) and is crucial for Th2 cell-mediated immune responses. In allergic diseases (such as asthma and atopic dermatitis), IL-4Rα plays a key role by promoting the production of IgE antibodies and the activation of inflammatory cells. Additionally, IL-4Rα is involved in the regulation of autoimmune diseases and the tumor microenvironment. Studying the signaling pathways and functional mechanisms of IL-4Rα helps to develop new therapeutic strategies for allergies, inflammation, and cancer. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD124, IL-4 receptor subunit alpha, Il4r, IL-4R subunit alpha, Il4ra FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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