Speedy™ Mouse RAGE One-Step ELISA Kit SE60031 proteintech
$449.00
In stock
SKU
SE60031
| Product name | Speedy™ Mouse RAGE One-Step ELISA Kit |
| Synonyms | Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL, 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 11596 |
| Sample Type | Average Range |
| Mouse serum | 112% 106%-116% |
| Cell culture supernatant | 106% 98%-117% |
| Tissue lysate | 88% 78%-103% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,034.6 16.0 1.5 |
| 2 | 16 254.2 4.2 1.7 |
| 3 | 16 132.3 3.0 2.3 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
RAGE (receptor for advanced glycation endproducts), also called AGER, is a transmembrane receptor of the immunoglobulin super family. It mediates interactions of advanced glycosylation end products (AGE) which accumulate in vascular tissue in aging in diabetes. Acts as a mediator of vascular inflammation such as atherosclerosis and a complication of diabetes. AGE/RAGE signal regulates production or expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation. Interaction with S100B after myocardial infarction regulates myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. ABPP-initiated RAGE signaling, especially stimulation of P38 mitogen-activated protein kinase (MAPK), delivers ABPP as a complex with RAGE to the intraneuronal. RAGE has higher expression in lung tissues, in particular in alveolar type I cells, and is lost in idiopathic pulmonary fibrosis (IPF). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Ager, Advanced glycosylation end product-specific receptor, MOK, RAGE 1, RAGE1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
