Speedy™ Mouse TNFRSF13B/CD267 One-Step ELISA Kit SE60065 proteintech
$449.00
In stock
SKU
SE60065
| Product name | Speedy™ Mouse TNFRSF13B/CD267 One-Step ELISA Kit |
| Synonyms | CD267, Taci, Tnfrsf13b, Transmembrane activator and CAML interactor, Tumor necrosis factor receptor superfamily member 13B |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 6.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 57916 |
| Sample Type | Average Range |
| Mouse serum | 105% 94%-117% |
| Cell culture supernatant | 106% 104%-110% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,301.5 59.8 2.6 |
| 2 | 16 506.9 13.2 2.6 |
| 3 | 16 261.0 5.2 2.0 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Tumor necrosis factor receptor superfamily member 13B (TNFRSF13B, also known as CD267) is a member of the tumor necrosis factor receptor superfamily. It is a receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS, binding both ligands with similar high affinity. TNFRSF13B plays a crucial role in humoral immunity by mediating activation of transcription factors and is involved in the stimulation of B- and T-cell function. It also promotes T-cell independent antibody responses and plasma cell differentiation, counteracting BAFF-driven B-cell activation. Additionally, the concentration of plasma TNFSF13B may be correlated with immune thrombocytopenia. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD267, Taci, Tnfrsf13b, Transmembrane activator and CAML interactor, Tumor necrosis factor receptor superfamily member 13B FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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