Speedy™ Mouse/Rat IGF-1 One-Step ELISA Kit SE70006 proteintech
$449.00
In stock
SKU
SE70006
| Product name | Speedy™ Mouse/Rat IGF-1 One-Step ELISA Kit |
| Synonyms | Igf1, IGF-I, Insulin-like growth factor I, Somatomedin |
| Reactivity | Mouse/Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma, Cell culture supernatant, Tissue lysate, Tissue homogenates |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 24482 |
| Sample Type | Average Range |
| Mouse serum | 95% 93%-97% |
| Rat serum | 94% 92%-97% |
| Cell culture supernatant | 91% 88%-94% |
| Tissue lysate | 95% 91%-98% |
| Tissue homogenates | 97% 91%-104% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 245.2 14.5 5.9 |
| 2 | 16 60.7 2.9 4.8 |
| 3 | 16 29.7 1.0 3.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone, is the principal mediator of biochemical effects of growth hormone (GH) and important in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas. Mice lacking IGF1 exhibit generalized organ hypoplasia including underdevelopment of the central nervous system and developmental defects in bone, muscle and reproductive systems. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Igf1, IGF-I, Insulin-like growth factor I, Somatomedin FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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