Speedy™ Mouse/Rat IGFBP2 One-Step ELISA Kit SE60032 proteintech
$449.00
In stock
SKU
SE60032
| Product name | Speedy™ Mouse/Rat IGFBP2 One-Step ELISA Kit |
| Synonyms | IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2 |
| Reactivity | Mouse/Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Mouse serum, Mouse plasma, Rat serum, Rat plasma |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16008 |
| Sample Type | Average Range |
| Mouse serum | 115% 110%-119% |
| Rat serum | 117% 112%-120% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 11.12 0.24 2.16 |
| 2 | 16 2.71 0.09 3.32 |
| 3 | 16 1.38 0.07 5.07 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
IGFBP2 (insulin-like growth factor-binding protein 2) is a secreted protein belonging to the IGF-binding protein family, which is widely involved in a variety of biological processes such as cell growth, differentiation, apoptosis and tumor progression. It regulates the activity of the IGF signaling pathway by binding to IGF-I and IGF-II, prolongs the half-life of IGF, and affects its bioavailability. IGFBP2 exhibits aberrant expression in a wide range of diseases, especially in tumors, where its high expression is closely related to malignant progression of tumors, metastasis and poor prognosis. In addition, IGFBP2 plays important roles in physiological processes such as nerve repair, energy metabolism and cell proliferation. For example, it promotes neuronal regeneration and glial cell proliferation, improves insulin resistance, and regulates the cell cycle. In the field of oncology, IGFBP2 can be used as both potential diagnostic marker and therapeutic target. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms IBP-2, IGF-binding protein 2, IGFBP-2, Insulin-like growth factor-binding protein 2, mIGFBP-2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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