Speedy™ Rat VEGFR-1/Flt-1 One-Step ELISA Kit SE70013 proteintech
$449.00
In stock
SKU
SE70013
| Product name | Speedy™ Rat VEGFR-1/Flt-1 One-Step ELISA Kit |
| Synonyms | Flt1, FLT-1, VEGFR-1, VGFR1 |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Tissue lyate |
| Assay type | Sandwich |
| Sensitivity | 10.8 pg/mL |
| Range | 156.3-10000 pg/mL, 78.1-5000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 54251 |
| Sample Type | Average Range |
| Rat serum | 86% 72%-95% |
| Tissue lysate | 105% 91%-123% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,366.0 42.3 1.8 |
| 2 | 16 611.5 21.9 3.6 |
| 3 | 16 309.7 15.0 4.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Soluble VEGF receptor-1 (sVEGFR-1, sFlt-1), a truncated version of the cell membrane-spanning VEGFR-1, has the potential to act as a decoy receptor for VEGFA. Elevated serum sVEGFR-1 have been found in women with preeclampsia. Excess placental sVEGFR-1 may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Flt1, FLT-1, VEGFR-1, VGFR1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent. 2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data? Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.
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