| Product name | Human NSE/ENO2 ELISA Kit |
| Synonyms | ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.11 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2026 |
| Sample Type | Average Range |
| Human plasma | 87% 71%-118% |
| Cell culture supernatants | 90% 70%-110% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 24 2.3 0.1 5.7 |
| 2 | 24 7.4 0.4 5.6 |
| 3 | 24 12.9 0.8 5.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | plasma Ann Surg MiR-7-5p as a Diagnostic and Monitoring Biomarker for Neuroendocrine Tumors: Insights from the SKY-NET Multicenter Study Authors - Yudai Higuchi View Article |
ENO2, also named as Enolases are cytoplasmic glycolytic enzymes that may be involved in differentiation. The enolase has three isoenzymes, alpha, beta and gamma. The alpha form is expressed in most tissues, whereas the beta form is expressed in muscle tissue. The gamma enolase (ENO2), particularly isoform neuron-specific enolase (NSE), a homodimer, is primarily localized in neurons and neuroendocrine cells and is a cancer diagnostic marker for brain tumors. ENO2 plays a role in the glycolysis-related energy pathway and might be involved in higher metabolic activity during the day than at night, at least in part. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms ENO2, NSE, 2-phospho-D-glycerate hydro-lyase, EC:4.2.1.11, Enolase 2 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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