| Product name | Mouse LIX/CXCL5 ELISA Kit |
| Synonyms | C-X-C motif chemokine 5,Cxcl5,Cytokine LIX,GCP-2(1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernates, Tissue lysates |
| Assay type | Sandwich |
| Sensitivity | 19.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20311 |
| Sample Type | Average Range |
| Mouse serum | 113% 102%-124% |
| Tissue lysates | 103% 85%-119% |
| Cell culture supernates | 106% 82%-113% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 787.0 50.9 6.5 |
| 2 | 24 180.4 8.4 4.7 |
| 3 | 24 31.4 1.8 5.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| mouse | tissue homogenates Med Nanodrug-bacteria conjugates-mediated oncogenic collagen depletion enhances immune checkpoint blockade therapy against pancreatic cancer Authors - Zhaoting Li View Article |
| FH Dan (Verified Customer) (02-27-2026) | Mice fed with normal chow or high fat diet serum collected. CXCL5 showed increased levels upon high fat diet. Applications: Other Cell Tissue Type: mouse serum |
LPS-induced CXC chemokine (LIX) is a murine chemokine similar to two human chemokines, ENA-78 (CXCL5) and GCP-2 (CXCL6). Mouse LIX can be produced by many immune cells, such as macrophages, eosinophils , as well as non-immune cells including mesothelial cells, and fibroblasts. Increased serum LIX has been observed in obesity and insulin resistance in mice and humans and is associated with complications of diabetes including nephropathy and atherosclerosis. It is mainly produced when induced by LPS, IL-17 and/or TNF-α. According to the previous studies, cancer cell autonomous secretion of LIX as sufficient to drive infiltration of mature, protumorigenic neutrophils in a mouse model of non-small cell lung cancer (NSCLC). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms C-X-C motif chemokine 5,Cxcl5,Cytokine LIX,GCP-2(1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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