Human APOE ELISA Kit KE01002 proteintech
$299.00
In stock
SKU
KE01002
| Product name | Human APOE ELISA Kit |
| Synonyms | Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva, Human milk |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 348 |
| Sample Type | Average Range |
| Human serum | 93% 85%-98% |
| Cell culture supernatant | 100% 95%-107% |
| Urine | 98% 94%-103% |
| Saliva | 94% 91%-99% |
| Human milk | 85% 79%-94% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 5.24 0.13 2.44 |
| 2 | 16 1.34 0.05 3.68 |
| 3 | 16 0.71 0.02 2.78 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
APOE (apolipoprotein E) is a plasma protein implicated in lipid metabolism. It is produced predominantly by hepatocytes, macrophages, and neural cells. In the CNS, APOE is the major extracellular lipid carrier and plays a key role in neuronal protection and repair after injury. APOE is polymorphic, with three major alleles: APOE2 (cys112, cys158), APOE3 (cys112, arg158), and APOE4 (arg112, arg158). APOE2 plays a protective role against both Alzheimer's (AD) and heart disease. APOE4 confers a higher risk for atherosclerosis and Alzheimer's disease. Analysis of cerebrospinal fluid (CSF) and plasma APOE protein levels in AD patients had been performed to examine its association with AD pathology. In old age, high plasma APOE levels precede an increase of circulating CRP and strongly associates with cardiovascular mortality. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Apo E, Apo-E, apoe4, apolipoprotein E, ApolipoproteinE FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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