Rat IL-10 ELISA Kit KE20022 proteintech
$399.00
In stock
SKU
KE20022
| Product name | Rat IL-10 ELISA Kit |
| Synonyms | Il10, IL10X, interleukin 10 |
| Reactivity | Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants,Tissue lysates,Tissue homogenizations |
| Assay type | Sandwich |
| Sensitivity | 6.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 25325 |
| Sample Type | Average Range |
| Rat serum | 82% 76-104% |
| Cell culture supernatants | 105% 82%-119% |
| Tissue lysate | 86% 76%-94% |
| Tissue homogenization | 101% 83%-120% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 1,792.2 72.9 4.1 |
| 2 | 24 435.4 9.2 2.1 |
| 3 | 24 113.1 3.7 3.2 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| rat | serum Front Pharmacol Investigating the regulation of the miR-199a-3p/TGF-β/Smad signaling pathway by BSHXF drug-containing serum combined with ADSCs for delaying intervertebral disc degeneration Authors - Enxu Liu View Article |
IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Il10, IL10X, interleukin 10 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Nanoparticles loaded with a CSF1R antagonist selectively depletes microglial cells and modulates inflammation in spinal cord injury
Journal: J Control Release | Authors: Jingjing Yang | Species: rat | Application: cell culture supernatants - An oral-gut microbial metabolite links Fusobacterium nucleatum to aggravated myocardial ischemia-reperfusion injury.
Journal: Gut Microbes | Authors: Yiwen Li | Species: rat | Application: Cell culture supernatant - Flowerbed-Inspired Mg-Loaded Scaffold Accelerates Critical-Sized Bone-Defect Repair by Reprogramming the Microenvironment.
Journal: ACS Nano | Authors: Xinyue Yang | Species: rat | Application: cell culture supernatants - Apigenin Alleviates Intestinal Ischemia/Reperfusion Injury via Upregulating Nrf2-Mediated Tight Junction Integrity
Journal: Mol Nutr Food Res | Authors: Bin Xu | Species: rat | Application: tissue homogenates - AD16 Modulates Microglial Activation and Polarization to Mitigate Neuroinflammation in Ischemic Stroke Models Through α7nAChR-ERK-STAT3 Signaling
Journal: CNS Neurosci Ther | Authors: Guo-Jian Zhao | Species: rat | Application: tissue homogenates - Investigating the regulation of the miR-199a-3p/TGF-β/Smad signaling pathway by BSHXF drug-containing serum combined with ADSCs for delaying intervertebral disc degeneration
Journal: Front Pharmacol | Authors: Enxu Liu | Species: rat | Application: serum
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