Human CNPY2 ELISA Kit KE00073 proteintech
$299.00
In stock
SKU
KE00073
| Product name | Human CNPY2 ELISA Kit |
| Synonyms | canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10330 |
| Sample Type | Average Range |
| Human plasma | 96% 85%-114% |
| Cell lysates | 88 85%-91% |
| Cell culture supernatants | 88% 80%-95% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 24 0.61 0.05 8.7 |
| 2 | 24 1.74 0.17 9.5 |
| 3 | 24 5.20 0.47 9.1 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | cell culture supernatants Front Pharmacol Liraglutide Increases VEGF Expression via CNPY2-PERK Pathway Induced by Hypoxia/Reoxygenation Injury. Authors - Chong Liu View Article |
CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth (PMID: 12826659). It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 is a HIF-1α regulated, secreted angiogenic growth factor that promotes smooth muscle cells migration, proliferation and tissue revascularization (PMID: 25589425). CNPY2 enhances cell spreading in fibroblasts and migration of rat C6 glioma cells (PMID: 15905959). CNPY2 was also shown to influence the expression of low-density lipoprotein receptor and FGF21 (PMID: 22378787). This kit is used to quantify CNPY2 level in vivo. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms canopy 2 homolog (zebrafish), CNPY2, CNPY2,MSAP, HP10390, MSAP, Protein canopy homolog 2, TMEM4, Transmembrane protein 4, ZSIG9 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Liraglutide Increases VEGF Expression via CNPY2-PERK Pathway Induced by Hypoxia/Reoxygenation Injury.
Journal: Front Pharmacol | Authors: Chong Liu | Species: human | Application: cell culture supernatants
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