Human Prolactin ELISA Kit KE00172 proteintech
$299.00
In stock
SKU
KE00172
| Product name | Human Prolactin ELISA Kit |
| Synonyms | PRL, prolactin |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Human milk |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5617 |
| Sample Type | Average Range |
| human serum | 99% 89%-110% |
| Cell culture supernatants | 94% 78%-109% |
| human milk | 103% 89%-123% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 72.9 4.1 5.1 |
| 2 | 24 264.1 7.1 2.7 |
| 3 | 24 958.0 28.2 2.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | Human milk Jpn J Nurs Sci A program for accelerating lactogenesis II using a breast pump shortly after cesarean section in Japan: A feasibility mixed-methods study. Authors - Ai Miyauchi View Article |
Prolactin (PRL) is a pleiotropic hormone which is mainly synthetized by the lactotrophs of the anterior pituitary gland. Prolactin has a wide variety of effects. It causes the breasts to grow and make milk during pregnancy and after birth. Prolactin levels are normally high for pregnant women and new mothers. Prolactin also plays an essential role in metabolism, regulation of the immune system and pancreatic development. Normally during pregnancy prolactin serum levels are increased to hold of insulin resistance (IR). As well, prolactin is involved in regulation of glucose metabolism, since high prolactin levels are associated with low incidence of type 2 diabetes mellitus (T2DM). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms PRL, prolactin FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- A Novel Method to Repair Thin Endometrium and Restore Fertility Based on Menstruation-Derived Stem Cell
Journal: Reprod Sci | Authors: Kai Chen - A program for accelerating lactogenesis II using a breast pump shortly after cesarean section in Japan: A feasibility mixed-methods study.
Journal: Jpn J Nurs Sci | Authors: Ai Miyauchi | Species: human | Application: Human milk - PGRMC1 suppression enhances progesterone responsiveness in stromal cells of ovarian endometrioma.
Journal: Reproduction | Authors: Yuko Izumi
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