Mouse DKK1 ELISA Kit KE10033 proteintech
$299.00
In stock
SKU
KE10033
| Product name | Mouse DKK1 ELISA Kit |
| Synonyms | Dkk1, mdkk 1 |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 31.25-2000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 13380 |
| Sample Type | Average Range |
| Mouse serum | 103% 80%-129% |
| Cell culture supernatant | 99% 79%-129% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 77.9 2.4 3.0 |
| 2 | 24 274.6 9.9 3.6 |
| 3 | 24 1,142.3 34.8 3.0 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Dickkopf 1 (DKK1), a foundational member of the Dickkopf proteins, has been identified as a naturally secreted glycoprotein that typically antagonizes the Wnt/β-catenin signaling pathway by sequestering the Wnt receptor complex LRP5/6 (PMID: 31335221). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PMID: 22738799). Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Dkk1, mdkk 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- DKK1+ tumor cells inhibited the infiltration of CCL19+ fibroblasts and plasma cells contributing to worse immunotherapy response in hepatocellular carcinoma
Journal: Cell Death Dis | Authors: Guangyu Fan
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