Mouse/Rat Osteopontin/OPN ELISA Kit KE10046 proteintech
$299.00
In stock
SKU
KE10046
| Product name | Mouse/Rat Osteopontin/OPN ELISA Kit |
| Synonyms | OPN, Osteopontin, 2AR, Bone sialoprotein 1, BSPI |
| Reactivity | Mouse/Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Urine, Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20750 |
| Sample Type | Average Range |
| Mouse serum | 88% 81%-95% |
| Mouse cell cuture supernatants | 103% 85%-120% |
| Mouse urine | 100% 80%-120% |
| Rat cell cuture supernatants | 93% 84%-108% |
| Rat serum | 87% 81%-93% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 27.6 1.6 5.8 |
| 2 | 24 114.4 4.2 3.7 |
| 3 | 24 444.4 17.4 3.9 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| mouse | serum Signal Transduct Target Ther Single-cell transcriptomic analysis reveals gut microbiota-immunotherapy synergy through modulating tumor microenvironment Authors - Minyuan Cao View Article |
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms OPN, Osteopontin, 2AR, Bone sialoprotein 1, BSPI FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Single-cell transcriptomic analysis reveals gut microbiota-immunotherapy synergy through modulating tumor microenvironment
Journal: Signal Transduct Target Ther | Authors: Minyuan Cao | Species: mouse | Application: serum - Senescence-associated tertiary lymphoid structures in Sjögren's disease model nishiura mice.
Journal: Mech Ageing Dev | Authors: Hiroshi Nishiura
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