Mouse/Rat Osteopontin/OPN ELISA Kit KE10097 proteintech
$299.00
In stock
SKU
KE10097
| Product name | Mouse/Rat Osteopontin/OPN ELISA Kit |
| Synonyms | AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1 |
| Reactivity | Mouse/Rat |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 6.3 pg/mL |
| Range | 39-2500 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 20750 |
| Sample Type | Average Range |
| Mouse serum | 97% 85%-112% |
| Rat serum | 100% 86%-130% |
| Cell culture supernatant | 106% 90%-123% |
| Mouse urine | 102% 91%-111% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 24 1,205.5 71.6 5.9 |
| 2 | 24 283.3 7.8 2.8 |
| 3 | 24 70.5 3.2 4.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| mouse | serum,cell culture supernatants Biochem Biophys Res Commun Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts Authors - Chao Meng View Article |
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidney, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling and is also involved in the inflammatory and immune response, oncogenesis, and cancer progression. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms AA960535, AI790405, Apl 1, BNSP, Bsp, BSPI, Eta, ETA 1, minopontin, OP, Opn, Opnl, Osteopontin, Ric, secreted phosphoprotein 1, Spp 1, Spp1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
- Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts
Journal: Biochem Biophys Res Commun | Authors: Chao Meng | Species: mouse | Application: serum,cell culture supernatants - Liposome-iRGD loaded with astragaloside IV alleviates the functional damage of endothelial progenitor cells via the Hippo-YAP/TAZ signaling pathway.
Journal: In Vitro Cell Dev Biol Anim | Authors: Churuo Zeng
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