Human ACSS2 ELISA Kit KE00857 proteintech
$299.00
In stock
SKU
KE00857
| Product name | Human ACSS2 ELISA Kit |
| Synonyms | ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.06 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 55902 |
| Sample Type | Average Range |
| Cell lysate | 78% 70%-91% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 10.14 0.59 5.82 |
| 2 | 16 2.58 0.11 4.11 |
| 3 | 16 1.36 0.05 3.70 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Acetyl-CoA synthetase 2 (ACSS2), an important member of the acetyl-CoA synthetase (ACSS) family, can catalyze the conversion of acetate to acetyl coenzyme A (acetyl-CoA). ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis. ACSS2 expression is highly correlated with nutrient deprivation, hypoxia, injury, immune activation, etc. ACSS2 is considered a target for tumor therapy and serves an important function in cell proliferation, gene expression regulation, and bioenergetics. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms ACAS2, AceCS1, Acetate--CoA ligase, Acetyl-CoA synthetase, Acetyl-CoA synthetase 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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