| Product name | Human ATG9A ELISA Kit |
| Synonyms | APG9L1, Autophagy-related protein 9A, mATG9 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.24 ng/mL |
| Range | 1.56-100 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 79065 |
| Sample Type | Average Range |
| Cell lysate | 94% 75%-112% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 51.22 1.60 3.12 |
| 2 | 16 13.97 0.59 4.24 |
| 3 | 16 7.40 0.46 6.21 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
ATG9A, a transmembrane protein of the core autophagy pathway, cycles between the Golgi, endosomes and a vesicular compartment. It is involved in autophagosome assembly and enables phospholipid scramblase activity. ATG9A is expressed ubiquitously across various tissues, with high expression in the brain and spinal cord. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms APG9L1, Autophagy-related protein 9A, mATG9 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.