Human CD80/B7-1 ELISA Kit KE00871 proteintech
$299.00
In stock
SKU
KE00871
| Product name | Human CD80/B7-1 ELISA Kit |
| Synonyms | CD80, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 11.5 pg/mL |
| Range | 78.1-5000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 941 |
| Sample Type | Average Range |
| Human serum | 100% 77%-129% |
| Cell culture supernatant | 97% 78%-123% |
| Urine | 104% 83%-126% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,637.9 99.1 3.8 |
| 2 | 16 643.2 23.5 3.7 |
| 3 | 16 318.9 11.9 3.7 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD80, also known as B7-1, is a type I membrane protein and a member of the immunoglobulin superfamily. It plays a crucial role in T cell activation and regulation by providing a co-stimulatory signal necessary for T cell proliferation and cytokine production. CD80 interacts with CD28 on T cells to promote T cell activation and with CTLA-4 to inhibit T cell activation. This dual role highlights its importance in modulating immune responses. Additionally, CD80 acts as a receptor for adenovirus subgroup B, indicating its involvement in viral infections. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD80, Activation B7 1 antigen, Activation B7-1 antigen, B7, B7 1 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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