Human CKAP4 ELISA Kit KE00620 proteintech
$299.00
In stock
SKU
KE00620
| Product name | Human CKAP4 ELISA Kit |
| Synonyms | 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 10970 |
| Sample Type | Average Range |
| Human serum | 93% 74%-104% |
| Cell lysate | 100% 83%-105% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 563.2 54.1 9.6 |
| 2 | 16 128.9 11.6 9.0 |
| 3 | 16 72.7 7.1 9.8 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation. In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2, and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63, p63 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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