| Product name | Human Collagen Type I ELISA Kit |
| Synonyms | COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.09 ng/mL |
| Range | 0.313-20 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1278 |
| Sample Type | Average Range |
| Human serum | 90% 86%-98% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 24 5.14 0.35 6.71 |
| 2 | 24 1.53 0.14 9.44 |
| 3 | 24 0.68 0.06 9.27 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| human | Serum, Plasma NPJ Sci Food From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients. Authors - Lorenza d'Adduzio View Article |
Type I collagen is the main structural component of connective tissues such as skin, tendon and bone, and it is the most abundant and widely expressed collagen in human body. Collagen type I is a heterotrimer, which contains one α2(I) and two α1(I) chains, which are encoded by unlinked loci COL1A2 and COL1A1, respectively. COL1A2 gene mutation is associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis and atypical Marfan syndrome. This product mainly recognizes the C-terminal propeptide. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms COL1, COL1A2, Alpha-2 type I collagen, col I, Collagen alpha 2(I) chain FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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