Human DNA2 ELISA Kit KE00654 proteintech
$299.00
In stock
SKU
KE00654
| Product name | Human DNA2 ELISA Kit |
| Synonyms | DNA2L, KIAA0083 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 52.6 pg/mL |
| Range | 78.1-5000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1763 |
| Sample Type | Average Range |
| Cell lysate | 94% 81%-111% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 2,741.0 263.5 9.6 |
| 2 | 16 558.6 26.6 4.8 |
| 3 | 16 274.6 15.2 5.5 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family, plays versatile roles in processing DNA intermediates during DNA replication and repair. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. DNA2 expression was significantly increased in human cancers and its expression correlated with patient outcome. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms DNA2L, KIAA0083 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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