Human E-cadherin ELISA Kit KE00818 proteintech
$299.00
In stock
SKU
KE00818
| Product name | Human E-cadherin ELISA Kit |
| Synonyms | Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324 |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.17 ng/mL |
| Range | 0.47-30 ng/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 999 |
| Sample Type | Average Range |
| Human serum | 97% 87%-106% |
| Urine | 105% 101%-110% |
| Saliva | 79% 73%-84% |
| Cell culture supernatant | 99% 97%-103% |
| Sample | n mean ( ng/mL ) SD CV% |
| 1 | 16 14.02 1.11 7.90 |
| 2 | 16 3.69 0.30 8.03 |
| 3 | 16 2.10 0.13 6.33 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
E-cadherin is a transmembrane glycoprotein that connects epithelial cells at adherens junctions. In normal cells, E-cadherin exerts its tumor-suppressing role mainly by sequestering β-catenin from its binding to LEF (Lymphoid enhancer factor)/TCF (T cell factor), which serves the function of transcribing genes of the proliferative Wnt signaling pathway. The loss of E-cadherin expression associated with the epithelial-mesenchymal transition (EMT) occurs frequently during tumor metastasis. Furthermore, Soluble E-cadherin (an 80-kDa soluble form) may be a biomarker for the diagnosis and prognosis of cancer. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms Arc 1, Cadherin 1, Cadherin-1, CAM 120/80, CD324 FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Reviews
Write Your Own Review